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di 2 ethylhexyl phthalate dehp c 24 h 38 o 4  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology di 2 ethylhexyl phthalate dehp c 24 h 38 o 4
Di 2 Ethylhexyl Phthalate Dehp C 24 H 38 O 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c 2 -ceramide  (Millipore)
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Millipore c 2 -ceramide
( A and B ) Representative electron micrographs of the kidneys ( A ) and the urine pellets ( B ) of nonobese and obese Tfeb fl/fl mice ( n = 2–3). ( C ) Urinary lipidomics of nonobese and obese mice ( n = 5 or 7). Data are presented as the total amount of each lipid species normalized to urine creatinine concentration. Bars: 5 μm ( A and B ) and 500 nm ( B , magnified image). Values represent means ± SEM. Statistically significant differences: # P < 0.05 versus nonobese mice ( C , 2-tailed Student’s t test). BM, basement membrane; MLB, multilamellar body; TL, tubular lumen; N, nucleus; BMP, bis(monoacylglycerol) phosphate; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; PG, phosphatidylglycerol; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; SM, sphingomyelin; Cer, <t>ceramide;</t> HexCer, hexose ceramide; LacCer, lactosylceramide; FA, fatty acid; MG, monoacylglycerol; DG, diacylglycerol; TG, triacylglycerol; CE, cholesterylester.
C 2 Ceramide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c 2 -ceramide  (Enzo Biochem)
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Mitochondrial translocation of TRIP-Br1 via PP2A-mediated dephosphorylation upon STS treatment. A Phosphorylation status of TRIP-Br1 was analyzed using a Phos-tag SDS-PAGE gel. Lysates of MCF7 cells were prepared and then exposed to CIP for 1 h at 37°C, followed by 8 % SDS-PAGE with 20 μM of phos-tag and immunoblotting analysis. Conventional SDS-PAGE was also performed with the same lysate as a control. B MCF7 cells were treated with 0.1 μM STS for 90 min followed by subcellular fractionation. Fractionated samples were subjected to Phos-tag SDS-PAGE. C MCF7 cells were treated with STS (0.1 μM) and/or okadaic acid (50 nM) for 3 h. Fractionated samples were subjected to western blot analysis. D U2OS 4.3 osteosarcoma cells were pre-incubated with or without doxycycline (1 μg/mL) to induce TRIP-Br1 expression for 24 h before treatment with okadaic acid (50 nM) for 24 h. E MCF7 cells were treated with okadaic acid (50 nM) for 16 h and incubated with Mitotracker (red) for 30 min. TRIP-Br1 (green) and Mitotracker (red) were visualized under a fluorescence microscope. F MCF7 cells were exposed to <t>C</t> <t>2</t> <t>-Ceramide</t> (10 μM) in a time-dependent manner followed by mitochondrial fractionation.
C 2 Ceramide, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nbd n 7 nitrobenz 2 oxa 1 3 diazol 4 yl c 6 ceramide  (Thermo Fisher)
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Mitochondrial translocation of TRIP-Br1 via PP2A-mediated dephosphorylation upon STS treatment. A Phosphorylation status of TRIP-Br1 was analyzed using a Phos-tag SDS-PAGE gel. Lysates of MCF7 cells were prepared and then exposed to CIP for 1 h at 37°C, followed by 8 % SDS-PAGE with 20 μM of phos-tag and immunoblotting analysis. Conventional SDS-PAGE was also performed with the same lysate as a control. B MCF7 cells were treated with 0.1 μM STS for 90 min followed by subcellular fractionation. Fractionated samples were subjected to Phos-tag SDS-PAGE. C MCF7 cells were treated with STS (0.1 μM) and/or okadaic acid (50 nM) for 3 h. Fractionated samples were subjected to western blot analysis. D U2OS 4.3 osteosarcoma cells were pre-incubated with or without doxycycline (1 μg/mL) to induce TRIP-Br1 expression for 24 h before treatment with okadaic acid (50 nM) for 24 h. E MCF7 cells were treated with okadaic acid (50 nM) for 16 h and incubated with Mitotracker (red) for 30 min. TRIP-Br1 (green) and Mitotracker (red) were visualized under a fluorescence microscope. F MCF7 cells were exposed to <t>C</t> <t>2</t> <t>-Ceramide</t> (10 μM) in a time-dependent manner followed by mitochondrial fractionation.
Nbd N 7 Nitrobenz 2 Oxa 1 3 Diazol 4 Yl C 6 Ceramide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n -[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-d- erythro -sphingosine (c 6 -nbd-ceramide  (Cayman Chemical)
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Mitochondrial translocation of TRIP-Br1 via PP2A-mediated dephosphorylation upon STS treatment. A Phosphorylation status of TRIP-Br1 was analyzed using a Phos-tag SDS-PAGE gel. Lysates of MCF7 cells were prepared and then exposed to CIP for 1 h at 37°C, followed by 8 % SDS-PAGE with 20 μM of phos-tag and immunoblotting analysis. Conventional SDS-PAGE was also performed with the same lysate as a control. B MCF7 cells were treated with 0.1 μM STS for 90 min followed by subcellular fractionation. Fractionated samples were subjected to Phos-tag SDS-PAGE. C MCF7 cells were treated with STS (0.1 μM) and/or okadaic acid (50 nM) for 3 h. Fractionated samples were subjected to western blot analysis. D U2OS 4.3 osteosarcoma cells were pre-incubated with or without doxycycline (1 μg/mL) to induce TRIP-Br1 expression for 24 h before treatment with okadaic acid (50 nM) for 24 h. E MCF7 cells were treated with okadaic acid (50 nM) for 16 h and incubated with Mitotracker (red) for 30 min. TRIP-Br1 (green) and Mitotracker (red) were visualized under a fluorescence microscope. F MCF7 cells were exposed to <t>C</t> <t>2</t> <t>-Ceramide</t> (10 μM) in a time-dependent manner followed by mitochondrial fractionation.
N [7 (4 Nitrobenzo 2 Oxa 1,3 Diazole)] 6 Aminocaproyl D Erythro Sphingosine (C 6 Nbd Ceramide, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ceramide (c-2  (Cayman Chemical)
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Cayman Chemical ceramide (c-2
Mitochondrial translocation of TRIP-Br1 via PP2A-mediated dephosphorylation upon STS treatment. A Phosphorylation status of TRIP-Br1 was analyzed using a Phos-tag SDS-PAGE gel. Lysates of MCF7 cells were prepared and then exposed to CIP for 1 h at 37°C, followed by 8 % SDS-PAGE with 20 μM of phos-tag and immunoblotting analysis. Conventional SDS-PAGE was also performed with the same lysate as a control. B MCF7 cells were treated with 0.1 μM STS for 90 min followed by subcellular fractionation. Fractionated samples were subjected to Phos-tag SDS-PAGE. C MCF7 cells were treated with STS (0.1 μM) and/or okadaic acid (50 nM) for 3 h. Fractionated samples were subjected to western blot analysis. D U2OS 4.3 osteosarcoma cells were pre-incubated with or without doxycycline (1 μg/mL) to induce TRIP-Br1 expression for 24 h before treatment with okadaic acid (50 nM) for 24 h. E MCF7 cells were treated with okadaic acid (50 nM) for 16 h and incubated with Mitotracker (red) for 30 min. TRIP-Br1 (green) and Mitotracker (red) were visualized under a fluorescence microscope. F MCF7 cells were exposed to <t>C</t> <t>2</t> <t>-Ceramide</t> (10 μM) in a time-dependent manner followed by mitochondrial fractionation.
Ceramide (C 2, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A and B ) Representative electron micrographs of the kidneys ( A ) and the urine pellets ( B ) of nonobese and obese Tfeb fl/fl mice ( n = 2–3). ( C ) Urinary lipidomics of nonobese and obese mice ( n = 5 or 7). Data are presented as the total amount of each lipid species normalized to urine creatinine concentration. Bars: 5 μm ( A and B ) and 500 nm ( B , magnified image). Values represent means ± SEM. Statistically significant differences: # P < 0.05 versus nonobese mice ( C , 2-tailed Student’s t test). BM, basement membrane; MLB, multilamellar body; TL, tubular lumen; N, nucleus; BMP, bis(monoacylglycerol) phosphate; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; PG, phosphatidylglycerol; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; SM, sphingomyelin; Cer, ceramide; HexCer, hexose ceramide; LacCer, lactosylceramide; FA, fatty acid; MG, monoacylglycerol; DG, diacylglycerol; TG, triacylglycerol; CE, cholesterylester.

Journal: JCI Insight

Article Title: TFEB-mediated lysosomal exocytosis alleviates high-fat diet–induced lipotoxicity in the kidney

doi: 10.1172/jci.insight.162498

Figure Lengend Snippet: ( A and B ) Representative electron micrographs of the kidneys ( A ) and the urine pellets ( B ) of nonobese and obese Tfeb fl/fl mice ( n = 2–3). ( C ) Urinary lipidomics of nonobese and obese mice ( n = 5 or 7). Data are presented as the total amount of each lipid species normalized to urine creatinine concentration. Bars: 5 μm ( A and B ) and 500 nm ( B , magnified image). Values represent means ± SEM. Statistically significant differences: # P < 0.05 versus nonobese mice ( C , 2-tailed Student’s t test). BM, basement membrane; MLB, multilamellar body; TL, tubular lumen; N, nucleus; BMP, bis(monoacylglycerol) phosphate; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; PG, phosphatidylglycerol; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; SM, sphingomyelin; Cer, ceramide; HexCer, hexose ceramide; LacCer, lactosylceramide; FA, fatty acid; MG, monoacylglycerol; DG, diacylglycerol; TG, triacylglycerol; CE, cholesterylester.

Article Snippet: To evaluate ceramide toxicity, PTECs were treated with C 2 -ceramide (MilliporeSigma, A7191) for 12 hours.

Techniques: Concentration Assay, Membrane

Mitochondrial translocation of TRIP-Br1 via PP2A-mediated dephosphorylation upon STS treatment. A Phosphorylation status of TRIP-Br1 was analyzed using a Phos-tag SDS-PAGE gel. Lysates of MCF7 cells were prepared and then exposed to CIP for 1 h at 37°C, followed by 8 % SDS-PAGE with 20 μM of phos-tag and immunoblotting analysis. Conventional SDS-PAGE was also performed with the same lysate as a control. B MCF7 cells were treated with 0.1 μM STS for 90 min followed by subcellular fractionation. Fractionated samples were subjected to Phos-tag SDS-PAGE. C MCF7 cells were treated with STS (0.1 μM) and/or okadaic acid (50 nM) for 3 h. Fractionated samples were subjected to western blot analysis. D U2OS 4.3 osteosarcoma cells were pre-incubated with or without doxycycline (1 μg/mL) to induce TRIP-Br1 expression for 24 h before treatment with okadaic acid (50 nM) for 24 h. E MCF7 cells were treated with okadaic acid (50 nM) for 16 h and incubated with Mitotracker (red) for 30 min. TRIP-Br1 (green) and Mitotracker (red) were visualized under a fluorescence microscope. F MCF7 cells were exposed to C 2 -Ceramide (10 μM) in a time-dependent manner followed by mitochondrial fractionation.

Journal: International Journal of Biological Sciences

Article Title: Inhibitory role of TRIP-Br1 oncoprotein in anticancer drug-mediated programmed cell death via mitophagy activation

doi: 10.7150/ijbs.72138

Figure Lengend Snippet: Mitochondrial translocation of TRIP-Br1 via PP2A-mediated dephosphorylation upon STS treatment. A Phosphorylation status of TRIP-Br1 was analyzed using a Phos-tag SDS-PAGE gel. Lysates of MCF7 cells were prepared and then exposed to CIP for 1 h at 37°C, followed by 8 % SDS-PAGE with 20 μM of phos-tag and immunoblotting analysis. Conventional SDS-PAGE was also performed with the same lysate as a control. B MCF7 cells were treated with 0.1 μM STS for 90 min followed by subcellular fractionation. Fractionated samples were subjected to Phos-tag SDS-PAGE. C MCF7 cells were treated with STS (0.1 μM) and/or okadaic acid (50 nM) for 3 h. Fractionated samples were subjected to western blot analysis. D U2OS 4.3 osteosarcoma cells were pre-incubated with or without doxycycline (1 μg/mL) to induce TRIP-Br1 expression for 24 h before treatment with okadaic acid (50 nM) for 24 h. E MCF7 cells were treated with okadaic acid (50 nM) for 16 h and incubated with Mitotracker (red) for 30 min. TRIP-Br1 (green) and Mitotracker (red) were visualized under a fluorescence microscope. F MCF7 cells were exposed to C 2 -Ceramide (10 μM) in a time-dependent manner followed by mitochondrial fractionation.

Article Snippet: Other reagents used in this study included staurosporine (STS) (A.G. Scientific, S-1016), chloroquine (CQ)(Sigma-Aldrich, C6628), okadaic acid (A.G. Scientific, O-1028), and C 2 -ceramide (Enzo Life science, BML-SL100).

Techniques: Translocation Assay, De-Phosphorylation Assay, SDS Page, Western Blot, Fractionation, Incubation, Expressing, Fluorescence, Microscopy